Fluid Dynamics Simulation, Molecular Simulation, Nano-computer Tomography, and Small Angle X-Ray Scattering to Understand Process Chromatography on a Nanoscale Level and to Predict Flow Pattern, Capacity and Resolution
Alois Jungbauer,Professor Department of Biotechnology, University of Natural Resources and Life Sciences Vienna, Austria Austrian Centre of Industrial Biotechnology
23rd February 2018, Friday 11:00AM, Seminar Hall, Dept of Chemical Engineering
Abstract:
The demanding requirements on quality of biopharmaceuticals can be only meet by a multi- step purification process. Chromatography is still the most important unit operation in purification of biopharmaceutics and is considered as the cost driver. In order to get a realistic picture on the mechanisms behind chromatographic separation and the effects of fouling and the possibilities to further improve productivity, the packed bed with its structure must be understood on a nanoscale level. The bed structure in monoliths and in chromatography columns packed with beads is in part responsible for flow non-uniformity. The structure also contributes to the pressure drop. It is well perceived that the structure close to wall is crucial for flow non-uniformity in chromatography columns. Computational fluid dynamics is one way to understand this flow non-uniformity and the pressure drop. Binding capacity can be only understood when we have a picture of the arrangement of proteins on the surfaces. In all cases we need an insight into the chromatography beds in nano-scale level. In this presentation two cases will be shown, first the flow properties of monoliths and packed beds by computational fluid dynamics based on serial block face scanning electron microscopy and a nano-computer tomography in a synchrotron and second the arrangement of antibodies on the surface of staphylococcal Protein A affinity chromatography media by molecular dynamics simulation based on SAXS measurement. These nanoscale methods will be discussed in context of current chromatography models and how this information can be used by the process engineer for process development and optimization.
Brief Bio:-
Professor Alois Jungbauer received his PhD in Food Technology and Biotechnology from the University of Natural Resources and Life Sciences Vienna, Austria 1986. He serves since then as a professor at the Department of Biotechnology. He teaches Protein Technology and Downstream Processing and Bioprocess Engineering. He also acts as area head and Dep. Director of Research in the Austrian Centre of Industrial Biotechnology. He is currently working in the field of bioengineering of proteins, plasmids and viruses with special focus on expression, downstream processing and characterization of large biomolecules.
As a proliferate researcher he has more than 300 publications on recombinant protein production and bioseparation, 15 patents and 12 book contributions and recently a monograph entitled “Protein Chromatography , Process Development and Scale Up”. He is executive editor and co-founder of Biotechnology Journal, and member of editorial boards from numerous journals in the area of biochemical engineering.